palmatine chloride hydrate  (Millipore)

Journal: Evidence-based Complementary and Alternative Medicine : eCAM

Article Title: Antioxidant and Anti-Inflammatory Activity Determination of One Hundred Kinds of Pure Chemical Compounds Using Offline and Online Screening HPLC Assay

doi: 10.1155/2015/165457

Figure Lengend Snippet: Free radical-scavenging capacities of antioxidant activity available measured with DPPH and ABTS assay on microwell plate.

Article Snippet: 74 , Palmatine chloride hydrate , Sigma , 257.82 , 2.57 ± 2.42 , 84.15 ± 2.43.

Techniques: Antioxidant Activity Assay, ABTS Assay, Concentration Assay

Journal: bioRxiv

Article Title: Identification of the myogenetic oligodeoxynucleotides (myoDNs) that promote differentiation of skeletal muscle myoblasts by targeting nucleolin

doi: 10.1101/2020.10.07.330472

Figure Lengend Snippet: Berberine enhances iSN04 activity by causing a shift in molecular structure. ( A ) The conformation of iSN04 simulated via TTP-McMD. ( B ) Contact map of iSN04. The scale indicates contact probability. ( C ) The simulated conformation of iSN04-berberine complex. Berberine is shown as a sphere model. ( D ) Contact map of iSN04-berberine complex. “B” indicates berberine. ( E ) Representative images of agarose gel electrophoresis of iSN04 mixed with berberine (Ber) in F10 medium. The EtBr-stained and UV-irradiated gel was scanned without filters (upper image) and with a 560-nm filter (lower image). ( F ) Ratio of MHC + cells within the mMBs treated with 10 μM iSN04 and 10 μM berberine in GM for 48 h (screening system). ** p < 0.01; NS, no significant difference (Scheffe’s F test). n = 3. ( G ) Ratio of MHC + cells within the mMBs treated with 10 μM of mutant iSN04 in GM for 48 h (screening system). * p < 0.05, ** p < 0.01 vs control; ‡‡ p < 0.01 vs iSN04 (Scheffe’s F test). n = 3. ( H ) Ratio of MHC + cells within the mMBs treated with 0, 1, or 3 μM iSN04 and 0, 1, 3, 10, or 30 μM of berberine in GM for 48 h (screening system). * p < 0.05 vs 0 μM-iSN04 + 0 μM-berberine; † p < 0.05, †† p < 0.01 vs 3 μM-iSN04 + 0 μM-berberine (Scheffe’s F test). n = 3. ( I ) Representative images of agarose gel electrophoresis of mutant iSN04 mixed with berberine in F10 medium. ( J ) Representative images of agarose gel electrophoresis of iSN04 mixed with coptisine (Cop), berberine, palmatine (Pal), or jatrorrhizine (Jat) in F10 medium. ( K ) Ratio of MHC + cells within the mMBs treated with 10 μM iSN04 and 10 μM berberine analogs in GM for 48 h (screening system). * p < 0.05 vs control-iSN04(-); †† p < 0.01 vs control-iSN04(+) (Tukey-Kramer test). n = 3.

Article Snippet: PS-ODNs, AS1411, berberine hydrochloride (Nacalai, Osaka, Japan), palmatine chloride hydrate (Nacalai), coptisine chloride (Wako, Osaka, Japan), and jatrorrhizine (Wako) were dissolved in endotoxin-free water.

Techniques: Activity Assay, Agarose Gel Electrophoresis, Staining, Irradiation, Mutagenesis, Control

Journal: The Journal of Biological Chemistry

Article Title: Identification of Plant-derived Alkaloids with Therapeutic Potential for Myotonic Dystrophy Type I *

doi: 10.1074/jbc.M115.710616

Figure Lengend Snippet: Representative qPCR splicing data for vehicle-treated WT and HSALR mice and compound-treated HSALR mice (quadriceps muscle). Shown is the percentage of exon 7a (CLCN1) and exon 22 (SERCA1) pre-mRNA inclusion. The alkaloids improve the splicing of CLCN1, whereas the splicing of SERCA1 is unchanged. a, DHB treatment. b, palmatine treatment. c, harmine treatment. d, alternative splicing of the CLCN1 pre-mRNA analyzed by RT-PCR and visualized on 3% agarose gel. Shown are two alternative splicing isoforms in vehicle-treated control mice and DHB-treated HSALR mice. **, p ≤ 0.01; ***, p ≤ 0.001.

Article Snippet: Berberine chloride (B3251), palmatine chloride hydrate (361615), and coralyne sulfoacetate (S424536) were obtained from Sigma-Aldrich.

Techniques: Reverse Transcription Polymerase Chain Reaction, Agarose Gel Electrophoresis